UC San Diego HUMANOID: Center of Excellence in Human Organoid Research

The UC San Diego Agilent Center of Excellence in Cellular Intelligence 's weekly series--#DidYouKnow--features examples of how you can push the limits of #Measurements-#Across-#Scales. This is our 15th post of this series. #DidYouKnow that #epithelial #morphogenesis requires a delicate balance between cell-cell #adhesion (via #junctions) and cell-#ECM interactions (via molecules such as #integrins). And if this balance is disrupted, it usually leads to epithelial reprogramming in ways to initiate almost all diseases we know (from #cancers, through #fibrosis to #diseases characterized by #inflammation). WHAT WAS DONE: Today's post (👇) continues to showcase how precise measurement of growing spheroids over ~10 days reveals how a mutant protein found in #cancers weakens cell-cell #adhesion and begins to grow in the form of tubes (arrows, bottom left) and that this feature can be corrected by adding #ECM (bottom right) such that #colonies grow as control (WT) #spheroids. HeLa cells were depleted of the WT endogenous protein (by CRISPR) and then transfected to select stable colonies of cells expressing the WT and the cancer-associated mutant protein. The mutant protein displays gain-of-function by being able to bind and modulate tyrosine-phosphorylated G proteins. This finding reveals a fascinating dynamic: with weaker junctions, the mutant cells became more reliant on cell-ECM interactions to regain their architecture. These results underscore the critical role of ECM not only as a structural scaffold but also as a mediator of cellular behavior in challenging conditions. Continuous #imaging and high-throughput [#HTP] exploration of all possible combinations in 96-well mode was key to revealing the importance of #balance to achieve #homeostasis in health, and how oncogenic mutants tip the same. What to know what #oncogene, and which #signaling pathways were the culprit? Stay tuned and follow our #publications. Post credit: Suchismita Roy and Kevin Perry If you want to know more about how xCELLigence RTCA eSIGHT can help with your research, contact us at: Kevin Perry [k3perry@health.ucsd.edu] Link to learn more about the eSIGHT: https://lnkd.in/gAzDis7 How to #access these and other #equipment: https://lnkd.in/g2iChuYf Need help scheduling? Kevin Perry [k3perry@health.ucsd.edu]. #training; #measurement; #biology; #quantitative; #imaging; #Oncogenesis #ExtracellularMatrix #CellAdhesion #BiomedicalResearch #EpithelialMorphogenesis; La Jolla Institute for Immunology Scripps Research UC San Diego

This holiday season, UC San Diego Institute for Network Medicine (iNetMed) is celebrating the intersection of science, art, and the boundless heart ❤️ of discovery 🔬. In the lab, where #creativity meets #precision, even a simple 3D spheroid can be programmed to lose #symmetry and take the shape of a #heart—reminding us that science isn’t just about solving problems; it’s about pursuing #questions with the #passion of an #explorer. When we #love what we do, every #discovery, big or small, becomes a #gift—not just to ourselves, but to the #world. Here's to the #joy of #discovery, the beauty of #curiosity, and the #heart that drives it all. Wishing you and yours a season filled with #wonder, #inspiration, and #love 💝. Photograph: Courtesy Suchismita Roy and Kevin Perry. What: We are looking at CRISPR-edited HeLa cells being grown as 3D spheroids in an RTCA eSIGHT at the UC San Diego Agilent Center of Excellence in Cellular Intelligence. #ScienceWithHeart ❤️🔬 #LoveWhatYouDo 💡💖 #SeasonOfDiscovery 🎁🌟

🌟 Meet Our Lab Stars: Agilent Instrument Edition 🌟 Meet the Agilent Cytation C10, a high-content imager that combines automated digital confocal and widefield microscopy with multimode microplate reading. This innovative design unlocks endless possibilities for cell analysis! Kevin Perry from the UC San Diego Agilent Center of Excellence in Cellular Intelligence affectionately calls his Cytation C10 “Cyclops.” Inspired by the X-Men character, “Cyclops” uses lasers for confocal imaging, just like the superhero’s laser vision. Join us in celebrating the incredible science at UC San Diego! 🚀 Discover the more unique names our customers have given to their Agilent instruments here: https://bit.ly/4fARMJt #GreatScience #LabStars #UCSD #Innovation

🌟 #Congratulations to Madhubanti Mullick on receiving a prestigious and Inaugural Postdoctoral Fellowship Award from the UC San Diego Agilent Center of Excellence in Cellular Intelligence! 🌟 Madhubanti’s research embodies the cutting edge of #interdisciplinary science, focusing on chasing #GPCRs in each cell of the #gut as #therapeutic #targets for #immunomodulation in #Inflammatory Bowel Disease (#IBD). With her extensive training in GPCR biology during her doctoral training and this exciting #fellowship, she’s poised to break new ground in this critical area of research. Using a comprehensive suite of high-throughput #technologies, Madhubanti will test GPCR modulation across various #cell types in the inflamed #colon. Her work is powered by a state-of-the-art #AI/ML model developed at the UC San Diego Center for Precision Computational Systems Network (PreCSN), which predicts with #mathematical precision the most promising targets from over 830 GPCR-target class candidates — pinpointing not just which GPCR to target (turns out, only a handful are worth it!), but also the specific #cell type and #MoA to focus on. Her studies at the UC San Diego Agilent Center of Excellence in Cellular Intelligence will integrate cutting-edge technologies to evaluate how #GPCR engagement influences disease-driving features in each cell type, providing novel insights into the precise mechanisms of GPCR #pharmacology. This research has the potential to #revolutionize #therapeutic strategies for #IBD and beyond. We are immensely proud of Madhubanti’s commitment to pushing scientific boundaries, and we can't wait to see the transformative impact her work will have on #immunology and #precisionmedicine. 👏 #PostdoctoralFellowship #GPCRResearch #IBD #PrecisionMedicine #AIinScience #WomenInSTEM #ResearchInnovation UC San Diego Postdoctoral Association

UC San Diego Institute for Network Medicine (iNetMed) is proud to present its latest reason to celebrate our "bootcamp" program: #Congratulations to Mahitha Shree Anandachar, a stellar second year graduate student from the UC San Diego @Biomedical Sciences (BMS) Graduate Program who is pursuing her doctoral thesis in the laboratory of Pradipta Ghosh, for being awarded a prestigious American Heart Association (AHA) Predoctoral Fellowship! Her research focuses on understanding how infections can reprogram innate immune cells' ability to handle #cholesterol, impacting the development of #atherosclerosis. More specifically, Mahitha’s work dives into the role of a fascinating new class of molecules called GEMs (Guanine-nucleotide exchange modulators), which coordinate #signaling downstream of #pathogen-sensing receptors like #TLRs. Turns out, that these molecules may hold the key to understanding the intricate "crosstalk" between #infection-induced #immune responses and cholesterol metabolism. By exploring these mechanisms, Mahitha’s studies aim to shed light on why and how our gut microbes influence our susceptibility to heart disease. Importantly, her research could also provide valuable insights into #therapeutic strategies to intercept harmful signals and pave the way for new #treatments. We are so proud of Mahitha’s innovative work and excited to see the impact her research will have on the fields of immunology and cardiovascular health. 👏 #ResearchExcellence #AHA #PredoctoralFellowship #HeartHealth #Immunology #Microbiome #CardiovascularDisease #WomenInSTEM

🌟 Meet Our Lab Stars: Agilent Instrument Edition 🌟 Annelies Ondersma from the UC San Diego Agilent Center of Excellence in Cellular Intelligence team is utilizing an Agilent LC/MS system they have called "Buzz" for her research in gastric cancer and mother infant metabolite studies. This instrument provides the precision, versatility, and sensitivity necessary for such a wide range of applications, some might say almost infinite! “We named the LC/MS Buzz because it is capable of parts per quadrillion level analyte detection,” said Annelise. “... and, true to the tagline of that [famous] space ranger, its applications in proteomics and metabolomics are nearly limitless.” Discover more Lab Stars from around the globe: https://bit.ly/3CNIEmd #GreatScience #LabStars #UCSD

🌟 Meet Our Lab Stars: Agilent Instrument Edition 🌟 With an Agilent Novocyte Penteon flow cytometer helping you do your cell analysis, the possibilities are endless! That’s why Ella from the UC San Diego Agilent Center of Excellence in Cellular Intelligence team, chose to call hers “Perchance” Why Perchance? Well, just as the word “perchance” suggests, the possibility of something wonderful happening. With its advanced capabilities, Perchance helps Ella and her team delve into the complexities of cellular intelligence, making groundbreaking discoveries in disease treatment and research. #GreatScience #LabStars #UCSanDeigo #Perchance

🌟 Meet Our Lab Stars: Agilent Instrument Edition 🌟 At Agilent, we love showcasing the stellar work of our partners and the innovative ways they use our instruments to push the boundaries of science. Today, we're thrilled to introduce Kennith Carpio from the UC San Diego Agilent Center of Excellence in Cellular Intelligence. Kenny is on a mission to explore phage clearance and test various compounds to treat diseases, focusing on inflammation and macrophages in different environments, including co-cultures with epithelial cells and specifically Crohn’s disease patients. Kenny's favorite instrument is the eSight xCelligence, which he has affectionately nicknamed Eve. Inspired by the character from Wall-E, with its sleek, xtra-terrestrial look and feel, Eve could easily be mistaken for a gadget from a sci-fi movie! Check out the video to see Kenny and Eve in action and discover how Agilent instruments are making a difference in the lab. #GreatScience #LabStars #UCSanDeigo #CrohnsDisease

We are hiring! We are looking for driven individuals with the desire to learn and work as a team. Click the link below to apply. https://lnkd.in/gzYtHVG8

The UC San Diego Agilent Center of Excellence in Cellular Intelligence 's weekly series--#DidYouKnow--features examples of how you can push the limits of #Measurements-#Across-#Scales. This is our 14th post of this series. #DidYouKnow that aging #myofibroblasts that refuse to die are the bad actors that propel tissue #fibrosis practically any organ in our body? To solve such a problem, we can cut #time, #cost, and #effort by getting the most out of any study if we can combine #precision grouping of patients and multiple real-time #readouts. For example, last week we posted real-time #impedance + #apoptosis (imaging Annexin V) assays that provide a #dynamic, label-free method to monitor #cell #states, #responses to #stimuli or #drugs, and #changing #fates amidst a population of cells. Today's post (👇) continues to analyze that same ongoing experiment, and thereby, 'milk the cow' (proverbial) as we added two other #readouts, cellular #senescence (shown today) and #cytokine studies (not shown on supernatants collected). WHAT WAS DONE: This is a a novel #target [discovered using #MachineLearning among other computational approaches at the Institute for Network Medicine (iNetMed)] that selectively decimates #myofibroblasts that propel fibrostenotic #CrohnsDisease is being tested. Repeated daily doses of the drug (arrows) gradually bring the initial high #impedance of the pathology driving #profibrogenic #myofibroblasts (pink/magenta) similar to that of th benign type (blue). UC San Diego HUMANOID: Center of Excellence in Human Organoid Research worked closely with UC San Diego Center for Precision Computational Systems Network (PreCSN) to conduct deep characterization of--what appears to be a surprisingly consistent (and meaningful)--two molecular subtypes! Publications will follow. But the #drugdiscovery #workflow seems to be all set for now! Post credit: Harrison Penrose, Courtney Tindle, Ella McLaren, Mason Hayashi and Kevin Perry If you want to know more about how xCELLigence RTCA eSIGHT can help with your research, contact us at: Kevin Perry [k3perry@health.ucsd.edu] Link to learn more about the eSIGHT: https://lnkd.in/gAzDis7 How to #access these and other #equipment: https://lnkd.in/g2iChuYf Need help scheduling? Kevin Perry [k3perry@health.ucsd.edu]. #training; #measurement; #biology; #quantitative; #imaging; #drugdiscovery; #IBD, #fibrosis, #Crohnsdisease, #Myofibroblasts; #therapeutics; #bioenergetics; #metabolism. Salk Institute for Biological Studies La Jolla Institute for Immunology Scripps Research UC San Diego