Amphasys│Bioprocessing’s Post

No dye? No label? And how do you tag it? Single cell analysis – Label free! Impedance Flow Cytometry (IFC) is a label free technology for single cell analysis. Unlike optical methods, IFC does not need labels, markers or fluorochrome dyes for the identification of viable and dead cells or to study cell metabolic and health status.   Impedance Flow Cytometry goes beyond cell counting and even beyond analysis of dead and viable cells. IFC allows to detect the health status of cells in fermentation processes or to monitor the metabolic status of cell cultures during the production of proteins or other metabolites. And that all without markers or dyes!   Interested in how it works without labels and dyes? More information about single cell analysis based on Impedance Flow Cytometry you can find here: https://lnkd.in/esv6767J   #flowcytometry #cellanalysis #labelfree

VWR Nano spectroscopy

#Article La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells by Sara Knezevic and Dalibor Stanković https://lnkd.in/gZSTGNMP   #MDPI #carbon #Biochemical #cell #biosensors #sensors #openaccess   #Abstract This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.

Understanding NO Conversion to S-Nitrosothiols at Heme Iron using our SX20 Sequential mixing 🔬 Why use sequential mixing? 🤔 The standard SX20 or SX20-LED is equipped with two drive syringes, such that two reagents may be rapidly mixed together immediately prior to reaching the instrument’s observation cell. This works well for experiments of this type. A + B → AB, where AB is a supramolecular or coordination complex or a ligand bound to a biomolecule or  A + B → C, where C is a new product of the reaction. The instrument's sequential mixing configuration enhances experimental versatility by accommodating multi-step reactions.  - With four syringes and two drive rams, this setup allows precise mixing of reagents in a staged manner.  - The first drive ram combines two reagents into an ageing loop. - A third reagent is introduced before the solution enters the observation cell. - The setup supports reaction sequences involving intermediates. - For quench-flow operations, the accessory replaces the standard stopped-flow cell. - It enables rapid mixing, incubation, quenching, and sample recovery, expanding experimental capabilities. 📖 Check out this publication using the SX20 with sequential mixing: https://lnkd.in/ewTdjDcQ Learn more about SX20: https://bit.ly/3TbTs1P #NitricOxide #Snitrosothiols #StoppedFlow #SequentialMixing

When choosing a method to examine binding interactions that involve intrinsically disordered proteins (IDPs), there are a few important factors to keep in mind. IDPs are sticky, prone to aggregation, and have other unique challenges that make them tough to work with. So what should you consider when selecting an affinity measurement technique for your IDP research? 1️⃣ Biophysical vs Biochemical Assays: Some IDPs like transcription factors don't have biochemical activity, so they can't be analyzed using biochemical methods. Biophysical assays are necessary to measure binding, plus they can provide insights into potential aggregation alongside binding data. 2️⃣ Direct vs Indirect Binding Assays: IDPs interact with small molecules, DNA/RNA, and other proteins, making it essential to use a method that can assess multiple types of interactions—both direct binding and displacement assays for multi-component systems. 3️⃣ In-solution vs Immobilization-based Assays: Immobilization can alter the conformational states of IDPs, leading to inaccurate results. In-solution assays enable measurements under near-native conditions, preserving the protein's natural structure and providing more reliable data, while also allowing for measurements in the presence of large aggregates. Are you working with IDPs? Learn more about how Spectral Shift technology can help: https://lnkd.in/gmvY5jaf

How to detect, count, and characterize particles in complex biological fluids such as unfiltered plasma, blood, and cell lysates? EOS Classizer™ ONE provides multiparametric calibration-free data on single particles based on their unique optical properties. Each particle population in a mixture of particle-particle or particle-biological corpuscles is classified, identified, and analyzed independently. Discover more about EOS Srl technologies at www.eosinstruments.com. (In the figure: aging of subvisible PLGA and PLGA+PEG particles for drug delivery incubated in unfiltered human plasma. EOS Classizer™ ONE enables the control of particle stability in real conditions to improve product formulation). #classizerone #eosinstruments #eosclouds #particleanalysis #particlesizing #particlecounting #spes #spestechnologies #nanoplastics

Discover how we've utilized #fluorescence polarization to detect small #molecules, like #Ochratoxin A, without altering the #aptamer or the target. With a detection limit of 5 nM, our 2008 study stands out for its innovative approach to leveraging #aptamers and labelled #oligos for the rapid, sensitive, and specific determination of small molecules. 📖 Read the entire study now! https://lnkd.in/gn9Ecm4m

#mdpisymmetry Check this newly published article "A Computational DFT Study of the Stereoinversion of Succinimide Residues Formed in Proteins and Peptides Catalyzed by a Hydrogen Phosphate Ion: An Unsymmetrical SE1 Mechanism" at https://brnw.ch/21wNWYu Author: Ohgi Takahashi #catalyst #densityfunctionaltheory

Today's #cyclodextrin from Christos Pliotas form The University of Manchester research reveals new insights into the activation kinetics of mechanosensitive channels. By mimicking tension through the sequestering of lipids from membranes, cyclodextrins enable the conversion of mechanical cues into electrical signals. The extent of MscS activation depends on the cyclodextrin-to-lipid ratio, with lipids being depleted slower when MscS is present. This has implications for the activation kinetics of MscS in different membrane scaffolds. Additionally, MscS transits from closed to sub-conducting state(s) before it desensitizes due to the lack of lipid availability in its vicinity required for closure. This approach allows for monitoring tension-sensitive states in membrane proteins and screening molecules capable of inducing molecular tension in bilayers. https://lnkd.in/dZWGVc3j #protein #membrane

📰"Chloroform alters electrical activity of proteinoid microspheres, mimicking neurons. Implications for bioelectronics and origins of life. #proteinoids #biomimetics" by Panagiotis Mougkogiannis and Andrew Adamatzky. 🔎Read the full paper here: https://lnkd.in/dTpXWYKy