iQue® High-Throughput Screening (HTS) by Cytometry’s Post

This application note describes methods for the characterization, successful maintenance, scale-up, and selection of ideal Induced pluripotent stem cells (iPSCs) using rapid expression profiling with minimal sample volumes of 10 μL on the iQue® Advanced Flow Cytometry Platform, and real-time imaging on an Incucyte® Live-Cell Analysis System for easy morphological analysis. https://buff.ly/4cUEere Sartorius #ipsc #stemcells #celltherapy #cellculture

Why we choose the MSCs //? Why not Embryonic Stem cell althouhg have many importance?/// Induced pluripotent stem cells (iPSCs), as adult cells with overexpression of pluripotency factors such as OCT4, NANOG, SOX2, c-Myc, KLF44, and more, can be highly similar to ESCs, with self-renewal properties, great differentiation potential, but might show genomic unstability. MSCs are multipotent, self-renewal, with easy accessibility and culturally expandable in vitro with exceptional genomic stability and few ethical issues, marking its importance in cell therapy, regenerative medicine and tissue repairment. #stemcell #Regenerativemedicine #MSCs

Join our November 6th webinar on automated solutions to enhance reproducibility and consistency in Induced Pluripotent Stem Cell (iPSC) research. Learn how the latest technologies can revolutionize stem cell culture, isolation, and expansion. Register now! CellCelector - Single Cell, Colony or Organoid Selection and Retrieval

Enhancing cell therapy by selecting cells with high extracellular vesicle secretion: Ke Cheng at Columbia University, Dino Di Carlo at UCLA and collaborators utilized nanovial technology to enrich millions of single cells based on EV secretion, applying this approach to select mesenchymal stem cells with high EV secretion for improved therapeutic treatment https://lnkd.in/gGt2FTNP They found that the selected MSCs exhibited distinct transcriptional profiles linked to EV biogenesis and vascular regeneration, and maintained high levels of EV secretion after sorting and regrowth. An article also authored by Doyeon Felis Koo, Xiao Cheng, Shreya Udani, SEVANA BAGHDASARIAN, Dashuai Zhu, Junlang Li, Brian Hall, Natalie Tsubamoto, Shiqi Hu and Jina Ko #extracellularvesicles #exosomes #MSCs #flowcytometry #bioengineering #Vesiculab

Stem cells are totipotent because they have capability to differentiate in any organs and tissue through the mitosis cell division. https://lnkd.in/dTWpeGEk

Growth factors are important supplements for cell culture. For example, bFGF is critical to maintain stem cell culture in an undifferentiated state. BDNF and GDNF are used to differentiate human pluripotent stem cell-derived neural progenitor cells into neurons. #arigo offers pure and high-quality active growth factor proteins that are ideal cell culture supplements.   Learn more at https://lnkd.in/eUBNhzPv   #Active_protein #Growth_factor

PharmaBio Corporation has been recognized as the Top Biotech Company in Japan by Life Sciences Review. PharmaBio pioneers regenerative medicine with PAL-222, a stem cell therapy restoring retinal function in severe myopia. Led by Dr. Hitoshi Kusano, the company excels in cell sheet technology, advancing global ophthalmic care and precision medicine. “Our mission is to restore retinal function and improve lives for those suffering from severe ocular conditions,” says Dr. Hitoshi Kusano, CEO of PharmaBio. The article will also be featured in the forthcoming special print edition of Biotech 2024. Read More: https://lnkd.in/gVy-2UuT #RegenerativeMedicine #MyopiaTreatment #OphthalmicInnovation #CellSheetTechnology #StemCellTherapy #VisionRestoration #PharmaBio #ClinicalTrials #HealthcareInnovation #FutureOfMedicine

Extracellular vesicles (EVs) are lipid enveloped nanoparticles that are naturally produced by cells and function in the intercellular transfer of biological material such as proteins, RNAs and metabolites. They have been shown to act in an autocrine and paracrine manner to alter the functions of local and distant recipient cells, with a growing body of evidence highlighting their wide-ranging functions in regenerative processes such as stem cell maintenance, tissue repair and immune modulation. Read more: https://lnkd.in/gpbqUu2H #Biomaterials #tissuerepair #extracellularvesicles #StemCells #kosheeka #ClinicalTrials #nanoparticles

Sometimes the optimal cell therapy requires a mixture of cell types. In a new study (https://hubs.li/Q02yV5mY0) from the Catholic University of Korea, a rat model of spinal cord injury showed that combination of human motor neuron progenitor cells (iMAP) and human mesenchymal stem cells (MSCs) yielded better structural and behavioral improvements compared to either cell type alone. The iMNP cells were generated from iPSCs using a cocktail of Stemgent small molecules. Find out how REPROCELL can help you with your multicell type cell models: https://hubs.li/Q02yVggx0

Read our latest article where Brandoch Cook, Ph.D., Senior Director, R&D at Enzo, discusses the potential of induced pluripotent stem cells (iPSCs) for cell replacement therapy and the use of small molecules to induce neural differentiation, reprogram cells into iPSCs, and convert mature somatic cells into neural stem and progenitor cells.     Read the article on https://lnkd.in/eJwUabKr #ScientistsEnablingScientists #celltherapy #smallmolecules #stemcells