Journal Biology of the Cell’s Post

The Oxford Organoid Hub will be launching in the Department of Physiology Anatomy and Genetics in the coming year. This is a combined effort with Filipa Simões with support from the British Heart Foundation Centre of Research Excellence. Please get in touch if you want to know more. https://lnkd.in/eySy8e6g Photo Credit: Cheryl Tan: A bio printed ventricular spheroid made from human iPSC derived cardiomyocytes.

Well, this was unexpected! 😲 Nanolive SA label-free video reveals bizarre Antimycin A-driven effects on Mitochondria. ⭕️⭕️⭕️ Recent advancements in live-cell imaging have unveiled astonishing details about mitochondrial dynamics, thanks to Nanolive’s label-free imaging technology. This cutting-edge approach, utilizing holotomography, allows researchers to observe living cells in three dimensions without the need for dyes, preserving cellular integrity and providing a more accurate physiological context. Nanolive’s label-free live imaging offers unparalleled insights into mitochondrial behavior, revolutionizing our understanding of cellular responses to stress and pharmacological agents. This technology is set to become an essential tool for cell biology research, providing new perspectives on mitochondrial physiology and pathology. #Nanolive #Mitochondria #LabelFree #Holotomography

#Cardiacorganoids (COs) are miniaturized and simplified organ structures that can be used in #heartdevelopment biology, #drugscreening, #diseasemodelling, and #regenerativemedicine. This cardiac organoid (CO) model is revolutionizing our perspective on answering major cardiac physiology and pathology issues. #VEGF is used in cardiac organoids to promote the #growth of #bloodvessels within these miniature heart structures. By adding VEGF to the #culturemedium, researchers can stimulate #angiogenesis and enhance vascularization, improving the oxygen and nutrient supply to the growing cells. This contributes to the development of more physiologically relevant and functional cardiac organoids for various applications. For more information on our #highlycited and #highquality #VEGF visit our website https://lnkd.in/e7XDkNAA or contact your local PeproTech/Thermo Fisher Scientific Representative! #PeproTech #ThermoFisherScientific #Gibco #GrowthFactors #Cytokines #CellCultureMedium #Angiogenesis #Heart #Organoids #VEGF #RUOGrade #AnimalFree #GMPGrade

#Tauopathies is a collective terminology for about 20 #NeurodegenerativeDisorders such as #Alzheimer’sDisease, #ProgressiveSupranuclearPalsy, #CorticobasalDegeneration. Tauopathies are defined by the abnormal deposition of hyperphosphorylated #Tau protein in the #brain. In Alzheimer’s disease (AD), in addition to intra-cellular deposition of tau, #amyloidβ peptides form extra-cellular #aggregates. The tau aggregates spread to other #neurons, causing neuronal loss and subsequent cognitive decline.   For treatment of tauopathies, the structures of purified protein aggregates have been used to design #drugs that disassemble the aggregates, preventing hyperphosphorylation or controlling the expression of tau among other strategies. Although several drugs were found to be promising during pre-clinical studies, most failed during #clinicaltrials. This is possibly due to the differences between #invitro and #invivo architectures of tau aggregates. Thus, it appears that there is an increasing need to obtain in vivo structures of tauopathy-related aggregates for effective #drugdesign.   Madeleine A. G. Gilbert and colleagues published an article on #bioRxiv detailing the #intissue #architecture of amyloid-β and tau aggregates. The authors employed cryo fluorescence microscopy (#cryoFM) and cryo focused ion beam milling (#cryoFIB) to obtain thin sections with regions of interest from the #postmortem #human #brain with AD. Electron cryotomography (#cryoET) revealed the interiors of brain sections and in situ structures of the tau and β-amyloid aggregates were determined by #SubtomogramAveraging. To learn more about their interesting findings, please go through the article linked below. https://lnkd.in/dRR-UvF3   Extending similar #invivo approach to investigate large number of diverse AD brains and different brain regions will be useful to obtain various structures of tau aggregates. The structural information will be valuable for developing #EffectiveDrugs to neutralize tau and related protein aggregates.   #alzheimersdisease, #tauopathies, #pathology , #NeurodegenerativeDisorders, #InVivoStudies

Doppler holography offers an in-depth view of deep choroidal vessels, crucial for nourishing the retinal pigment epithelium and photoreceptors, and is increasingly significant in retinal disease research. Traditional methods struggle with the choroid's complex anatomy, but Doppler holography provides high-contrast imaging that rivals or surpasses techniques like ICG-A and OCT. It differentiates choroidal arteries from veins, revealing structures such as arteries near the optic nerve and para-optic short posterior ciliary arteries, while visualizing more submacular arteries than ICG-A, especially in the early arterial phase. Its wide-field imaging captures vortex veins beyond the posterior pole, making this noninvasive method invaluable for studying choroidal anatomy and physiology.

Detection of heat shock proteins 70 in the gill, liver, and cardiac muscle of Carassius auratus with confocal microscopy https://t.co/IGeGchaY4S #EurekaMag

New research has uncovered an exciting discovery about the regulation of mitochondrial morphology in dendrites of neurons in vivo. The study highlights a signaling pathway that involves activity-dependent regulation of mitochondria fission/fusion balance through the activation of AMPK. This activation occurs via the Ca2+ and Camkk2-dependent phosphorylation of two direct effectors: the pro-fission Drp1 receptor Mff and the recently identified anti-fusion, Opa1-inhibiting protein, Mtfr1l. The findings provide insight into the compartment-specific morphology of dendritic mitochondria, shedding light on the importance of activity-dependent regulation. Check out the full article for more details! #neuroscience #mitochondria #research

📍 Immunoglobin-associated senescence as a hallmark of aging   Ma et al. produced an in-depth spatial transcriptomic profile of nine tissues in male mice during aging to systematically characterize the loss of tissue integrity and organ dysfunction resulting from aging.   Highlights 🔹 Spatial transcriptomics shows increased entropy in tissue structure in aging male mice 🔹 Senescence-sensitive spots are characteristic features of tissue aging 🔹 Immunoglobulin G accumulates in various tissues of mice and humans during aging 🔹 Immunoglobulin-associated senescence might be a target for geroprotective intervention ➡️ More details: https://lnkd.in/gr6vgAVa #ChineseAcademyofSciences #spatialomics #spatialbiology #singlecellanalysis #singlecell

Excited to announce our latest publication (one of my PhD projects) in #NatureCommunications (Nature Portfolio) titled "Atomistic mechanism of coupling between cytosolic sensor domain and selectivity filter in TREK K2P channels"! Check it out here: https://lnkd.in/eU5GP_4g Our integrated approach, combining #electrophysiology, #mdsimulations, interaction network analysis, systematic cysteine scanning mutagenesis, lipid tethering and blocker experiments sheds light on the essential role of the pCt (proximal C-terminus) domain in TREK channel gating. We revealed how the interaction between the pCt domain and the membrane influences the conformational equilibrium between the up- and down-state of TREK channels. Furthermore, MD simulations proposed an allosteric mechanism at the atomistic scale, delineating two pathways of the energetic coupling between the cytosolic sensing domain and the selectivity filter. We also provided atomistic insights into the physiological regulation of TREK channels by phosphorylation. #ionchannels #moleculardynamics #structuralchemistry #computationalbiophysics #biophysics

#citation Did you know that motoneurons rely on precise protein synthesis for proper axon growth and neuromuscular connectivity? 🧠 🧬 Recent findings on 𝙉𝙖𝙩𝙪𝙧𝙚 𝘾𝙤𝙢𝙢𝙪𝙣𝙞𝙘𝙖𝙩𝙞𝙤𝙣𝙨 from Universitätsklinikum Würzburg reveal a key player—hnRNP R—that regulates axonal protein production. In knockout models, reduced axon growth and impaired motor function were observed. This discovery highlights the critical role of hnRNP R in controlling the local production of essential proteins for healthy motor behavior. Restoring axonal O-GlcNAc levels can rescue these defects! Read more here: https://lnkd.in/d2Gz8UtB Cyagen is proud to contribute to this groundbreaking research with our 𝘏𝘯𝘳𝘯𝘱𝘳 knockout mice. 🐭 Learn more about our Knockout Catalog Models here: https://lnkd.in/gqxybj-x #Neuroscience #Motoneurons #ProteinSynthesis #AxonGrowth #NeuromuscularResearch #ScientificDiscovery #knockoutmice #mousemodel #biotech #biotechnology #scienceofreading