MIMETAS’ Post

In vitro models, ex-vivo cultures, organ-on-chip, organoids, tissue engineering... do you know the difference between all of these terms and how they relate to drug discovery? 🤔 🚫 If not, have a look at our review about it and get all cleared out. 😎 ✅ If yes, check it out anyway, maybe you will learn something new! 😉 https://lnkd.in/ehBWmFu5 #organonchip #microfluidics #drugdiscovery #scientificdissemination

Dive into our latest blog where we dissect the diverse response criteria based on imaging endpoints. We’re comparing their pros and cons and exploring how they can be improved. Focusing on structural imaging like CT and MRI versus functional imaging in PET scans, we explore how each method may impact the evaluation of new medical treatments. Click here to read: https://lnkd.in/ggQi_reM #ImaginAb #immunooncology #CD8ImmunoPET #biotech #pharma #theranostics #PETCT #nuclearmedicine #clinicaltrials Matthew Hoover

Peptide and PEI(Polyethyleneimine)modified carbon quantum dots represent a versatile nanomaterial with potential applications in various fields including bioimaging, drug delivery,and biomedical diagnostics.modified carbon quantum dots can be utilized as fluorescent probes for cellular and tissue imaging due to their excellent photostability and biocompatibility. The inclusion of peptides in the modification can enable targeted imaging of specific biomolecules or cellular structures, enhancing their specificity. #Peptide #PEI #Polyethyleneimine #fluorescent #probes #diagnostics #enable https://lnkd.in/g_xZRs5D

Modification for Targeted Drug Delivery • Surface functionalization: Quantum dots can be functionalized with ligands or biomolecules to specifically bind to target cells or tissues, enabling targeted drug delivery and imaging. • Encapsulation: Quantum dots can serve as carriers to encapsulate and protect drugs, enhancing their stability and controlled release at the desired site.

Proud parent moments: 1st steps, words, school & job. Our own little baby, #QPatchCompact, made us v proud: data for 1st paper. Is it dusty in here? Think there's something in my eye. #patchclamp #planarpatch #ephys #electrophysiology #ephys #ionchannels #automatedpatchclamp

Lyophilized, highly purified Type I atelocollagen from Promed Bioscience Promed’s Type I atelocollagen has superior functionality and physical and mechanical properties (such as tensile strength and elasticity). It can be used for in vitro cell cultures, cell functional assays and soft 3D cell cultures. Find out more: https://lnkd.in/eez-narK Promed’s proprietary enzyme-based extraction method removes telopeptides at the non-helical N and C termini, resulting in a triple helix configuration that exhibits reduced immunogenicity. Why choose Type I atelocollagen from Promed? ✅ Lyophilized collagen – exhibits structural rigidity and stability. ✅ Collagen for gel formation, ideal for cell culture. ✅ Collagen fibrils retain mechanical strength and elasticity. ✅ Pore sizes ideal for tissue engineering and drug delivery. Browse atelocollagen products on MyBio: https://lnkd.in/eez-narK #atelocollagen #type1atelocollagen #porcineatelocollagen  #cellculture #collagen #medicalgradecollagen #collagenscaffolds

Tracking small extracellular vesicles using a PicoGreen labeling strategy: in their latest work, Sagar Rayamajhi, Andrew Godwin at University of Kansas Medical Center and collaborators demonstrated that PicoGreen, a fluorescent molecule that binds specifically to double-stranded DNA and emits at 520 nm, can effectively label dsDNA associated with small EVs to form a stable, highly fluorescent PG-DNA complex within EVs. In both 2D cell culture and 3D organoid models, PG-EVs exhibited efficient tracking capabilities, characterised by a high signal-to-noise ratio, uptake that was both time- and concentration-dependent, and the ability to navigate through 3D environments https://lnkd.in/eQdUMtA2 They further validated PG-EV tracking by using dual-labeled EVs with two complementary labeling methods: surface amine bioconjugation and endogenous mCherry-tetraspanin reporter protein expression in donor cells. An article co-authored by Benjamin Gibbs, PhD, Jared Sipes, Harsh B. Pathak and Stefan Bossmann #extracellularvesicles #exosomes #EVuptake #DNA #confocalmicroscopy #Vesiculab

Long-acting injectables were one of the hottest topics at last week's PODD. There are a lot of questions around LAIs, from connecting formulations with PK to building IVIVC models, these many unknowns require in depth research and understanding. Imaging is uniquely suited to support these. With X-ray micro-CT, the structure of in situ formed depots and nanosuspensions can be studied directly, either in vitro or within ex vivo tissue. Most critical, with advanced microstructure quantification and image-based simulation, hypotheses around the mechanism of release and impact of in vivo environment can be evaluated. If solubility driven release is suspected, in silico drug release evaluation can be used to compare this release mechanism against in vitro and in vivo data. If polymers are impacting release, studies of time-points can be used to quantify polymer degradation and other behaviors. In the example below, we compare the morphology of two in situ formed depots, where the mixing has profoundly impacted the shape and available surface area for dissolution. Together with our partners these workflows for critical formulation and pipeline decisions. Greater product understanding and use of digital release models is also a pathway to reduce the number on needed in vivo tests, and give greater confidence in clinical performance. 

Reversing the surface charge of exosomes to improve their drug delivery potential: in their latest study, Ambika Goel Bajpayee at Northeastern University and collaborators engineered charge-reversed cationic exosomes for mRNA delivery by incorporating cartilage-targeting arginine-rich cationic motifs into the exosome bilayer, utilizing buffer pH as a switch to reverse the charge https://lnkd.in/egfAjVu4 Positively charged exosomes penetrated the full thickness of early-stage arthritic human cartilage by forming weak and reversible bonds with glycosaminoglycans , enabling efficient delivery of encapsulated eGFP-mRNA to chondrocytes in deeper tissue layers, a capability not observed with unmodified negatively charged exosomes. An article also authored by @Chenzhen Zhang, Tanvi Pathrikar, Helna M. Baby, Jun Li, Hengli Zhang, Andrew Selvadoss, Arina Ovchinnikova, Andreia Ionescu, SUSAN Chubinskaya and Rachel E. Miller #extracellularvesicles #exosomes #bioengineering #drugdelivery #nanomedicine #Vesiculab

This article examines hydroxyl dendrimers targeting reactive microglia in cerebral palsy, revealing differential uptake that aids in designing targeted nanomedicines for neuroinflammation. You can check it out here: https://lnkd.in/gT9Ktakn #nanotechnology #biotechnology #pharmaceuticals #science #Innovation #research #healthTech #medicalDevices #biomedical #clinicalTrials #drugDelivery #precisionMedicine #healthcareInnovation #inflammation