Thomas Carrell’s Post

Nice work out of Karolinska University Hospital. "Mass spectrometry imaging (MSI) directly detects analytes from a surface, which enables spatial mapping of oxylipin biosynthesis and migration within tissue. MSI has lacked the sensitivity to routinely detect low abundant oxylipins; however, new multiple-reaction-monitoring (MRM)-based MSI technologies provide increased sensitivity. In this study, we developed a workflow to apply desorption electrospray ionization coupled to a triple quadrupole mass spectrometer (DESI-MRM) to spatially map oxylipins in pulmonary tissue....This study realizes the potential of targeted-MSI to routinely map low abundant oxylipins with high specificity at scale." The work is in pre-publication and is available from the ChemRxiv site: https://lnkd.in/gVV4q5UF #MSI #oxylipins #asthma #biomedicalresearch #MS4BetterCare

📚 Seeing is believing: Download this complimentary Article Collection today! 🔬 Imaging is one of the foundational techniques used in the life sciences. From imaging the expression of individual proteins or genes in single cells to the screening of drugs in multi-well plates, microscopy platforms for cellular imaging are integral tools for unravelling the complexities of biological systems. 🙋♀️ This free article collection from Wiley showcases a diverse range of studies that employ our EVOS Cell Imaging Systems (Thermo Fisher Scientific). What You Will Learn: 👉 Exploring K5 peptide’s role in improving protein delivery into cells 👉 Identifying cell receptors using fluorescent ligands 👉 Assessing crosslinking effects on collagen-elastin scaffolds for wound healing 👉 Investigating miRNAs’ effects on GCTB cell proliferation and movement 👉 Versatility of EVOS Cell Imaging Systems across multiple research fields 🤓 More info: https://lnkd.in/epkBGb5u #EVOS #imaging #thermofisherscientific

Nature / Nature Methods announced their method of the year was Spatial Proteomics. Spatial proteomics is an umbrella term that covers a broad swath of immunohistochemistry-based methods including: cyclic immunofluorescence (cycIF) co-detection by indexing (CODEX) iterative bleaching extends multiplexity (IBEX) multiplexed ion beam imaging (MIBI) imaging mass cytometry (IMC) Always pleasing to see the developments in Omics, the increase of coverage and reputation among many researchers.

Seeing is Believing: The Power of EVOS Imaging Systems in Uncovering Biological Insights. New Article Collection now available. Imaging is one of the foundational techniques used in the life sciences. From imaging the expression of individual proteins or genes in single cells to the screening of drugs in multi-well plates, microscopy platforms for cellular imaging are integral tools for unravelling the complexities of biological systems. What You Will Learn: - Exploring K5 peptide’s role in improving protein delivery into cells. - Identifying cell receptors using fluorescent ligands. - Assessing crosslinking effects on collagen-elastin scaffolds for wound healing. - Investigating miRNAs’ effects on GCTB cell proliferation and movement. - Versatility of EVOS Cell Imaging Systems across multiple research fields. Access your copy here: https://lnkd.in/eDfVKc_N Thank you to Thermo Fisher Scientific for your trusted Wiley support and partnership. #ImageAnalysis #miRNA #CellAnalysis #Microscopy #DrugDiscovery #ProteinScience #CurrentProtocols #LearnWithWiley

Resolving extracellular vesicles heterogeneity using single-vesicle flow cytometry: Di Vizio Dolores at Cedars-Sinai, Andries Zijlstra at Vanderbilt University/Genentech and collaborators introduced a technique called "EV Fingerprinting" to differentiate various vesicle populations by computing multiparametric data obtained from quantitative single-EV flow cytometry https://lnkd.in/eHVgd-qH Subsequent multiplexing experiments demonstrated that EV cargoes exhibit a specific, nonrandom distribution, with CD63 and CD81 preferentially partitioning into smaller and larger EVs, respectively. These findings not only offer a means to investigate EV biogenesis but also illustrate how selective cargo partitioning contributes to EV heterogeneity. An article also authored by Ariana von Lersner, Fabiane Fernandes, Patricia Midori Murobushi Ozawa, Ph.D., Marques Jackson, Matthieu Masureel, Hoangdung Ho, Sierra M. Lima, Tatyana Vagner, Bong Hwan Sung, Mohamed Wehbe, Kai Franze, Heather Pua, John Tanner Wilson, Jonathan Irish and Alissa Weaver #extracellularvesicles #exosomes #flowcytometry #biogenesis #biomarkers #Vesiculab

For those that are unfamiliar with MIBI, this technology is truly set apart from all other tissue imaging platforms. I know that buzzwords like “actionable insights” and “accelerate research” are ubiquitous in this space, but I assure you, If tissue samples are integral in your research or product development, then you’ll want to have a conversation with Matt and his team to learn about what MIBI can do for you.

🎊 Welcome back, #CuriousMinds! It's time for our weekly A-Z of Super-Resolution! Today, join us for a spotlight on "J" as we center our attention on Janelia Fluor dyes with Halo-tag. ✨ 🔬 Halo-tag is a strategy to specifically label proteins of interest for a variety of applications. The Halo-tag can bind multiple different ligands, among them different fluorophores, such as Janelia FluorⓇ. The first step is to use molecular biology to generate a fusion protein of the Halo-tag and the protein of interest. Next, the cells are supplemented with the ligand of choice, which will bind the fusion protein quickly, strongly, and specifically. In super-resolution microscopy, the key application is to fluorescently tag proteins for live-cell imaging or for fixed cells, in case it is impractical to use specific antibodies. 🕵️♀️💡 Ready to uncover more insights, #CuriousMinds? Stay tuned for a weekly dose of Super-Resolution wisdom! 🚀🔬 #SuperResolution #EnablingScientificExploration #Microscopy Missed the Super-Resolution Word of the Week from the past weeks? 🤔 Catch up now! https://hubs.li/Q02ntKqS0

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging #thermofisherscientific

Microflow workflow now also benefit from the timsTof’s unique combination of sensitivity, selectivity, speed and robustness

When the right ion optics work in harmony, one can dissect biology with highest efficiency using mass spectrometry. Over the years our proteomics community has come up with wonderful PASEF melodies to orchestrate symphonies of biomolecules. Marta Mendes, Klara Florentine Borrmann, and Gunnar Dittmar just published a great review on PASEF. It's a great read for existing and new #timsTOF users that are curious about unique #PASEF capabilities. "With the introduction of PASEF in combination with fast TOF systems and the switch from DDA to DIA, a big step toward full proteomes has been made. Currently, we witness how these improvements are translating into clinical research." What will be your symphonies of biomolecules? #StayCurious Shourjo Ghose Ph.D. Pierre-Olivier Schmit Torsten Müller Dijana Vitko https://lnkd.in/g5TfQRdC