Francesca Grespi’s Post

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimised for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

M&Ms anyone? Oh no, not the chocolates 🍫 Dr. Bendall and his team at Stanford recently presented a poster at ASMS 2024 about "MnM" — an innovative bimodal imaging strategy🔬 that combines mass spectrometry imaging (MALDI), high-definition spatial proteomics (MIBI), and H&E staining on the same tissue section. This technique is enabled by Ionpath’s new MIBIslide Blue and, in this study, allowed researchers to obtain data on cellular phenotype and activity (antibody-based MIBI) with post-translational and metabolic information (de novo MALDI) at the single cell level. Check out the poster for more info: https://hubs.la/Q02_mzkP0 #Proteomics #SpatialBiology

📍 Spatial proteomics as Method of the Year by Nature Methods   Spatial proteomics is an umbrella term that covers a broad swath of immunohistochemistry-based methods including, but not limited to, cyclic immunofluorescence (cycIF), co-detection by indexing (CODEX), iterative bleaching extends multiplexity (IBEX), multiplexed ion beam imaging (MIBI) and imaging mass cytometry (IMC).    ➡️ More information about spatial proteomics and the special issue via https://lnkd.in/epNmXHh9 #spatialomics #spatialbiology #singlecellanalysis #singlecell

📍 Integration of mass cytometry and mass spectrometry imaging for spatially resolved single-cell metabolic profiling Nunes et al. developed a novel multimodal mass spectrometry imaging approach for the integrated analysis of metabolites and immunophenotypes in human tissues.   Key points 📌 Integrating matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) and imaging mass cytometry (IMC) 📌 A wet-lab protocol allowing the application of both technologies on the same tissue section 📌 A data integration strategy enabling the relative quantification of metabolites (measured by MALDI-MSI) at the single-cell level (defined by IMC). ➡ More details: https://lnkd.in/emuRX48j #LeidenUniversityMedicalCenter #spatialomics #spatialbiology #singlecellanalysis #singlecell

How can BeatBox contribute to advancing single-cell tissue proteomics studies? In this exciting publication, scientists from the lab of Prof. Matthias Mann at the Max Planck Institute of Biochemistry describe a method called single-cell Deep Visual Proteomics (scDVP). This technology integrates high-content imaging, laser microdissection, and multiplexed ultra-sensitive mass spectrometry. The authors demonstrate that #scDVP resolves the spatial proteome of hepatocytes, paving the way for single-cell proteomics in complex tissues. We were delighted to see our BeatBox in support of their multiplexed DIA approach! 📚 Learn more: https://ow.ly/jRGH50T6iz0 #proteomics #LCMS #MassSpec #tissueprep #samplepreparation #singlecell #hepatocytes #DataSnippets #PreOmics

In the publication, "Epigenetic Alterations Associated with Dexamethasone Sodium Phosphate through DNMT and TET in RPE Cells," researchers utilized the ISS AlbaSTED (FLIM/FFS Laser Scanning Microscope) for: ✨ Stimulated Emission Depletion (STED) Microscopy 🔍 Confocal Imaging 🧬 Fluorescence Correlation Spectroscopy (FCS) This sophisticated instrumentation enabled researchers to achieve unprecedented insights including how dexamethasone (DEX) sodium phosphate affects epigenetics. Key findings include: 🧬 DEX causes DNA hypomethylation & hyper-hydroxymethylation 🧫 Altered mRNA levels of DNMT & TET proteins ⚙️ DEX hinders DNMT activities These results highlight the critical role of DNMT in DEX-induced epigenetic changes. Dive into the details here: https://lnkd.in/duvuuVK4 AlbaSTED: https://lnkd.in/dAeUwN_e #Epigenetics #Research #DEX #ISS #ImagingTech #ScienceInnovation #confocalimaging #STED #FCS #dna