Vesiculab’s Post

One step closer to long-term vascularization of 3D in vitro models? 📢 🎉 Our new paper is out in Advanced Functional Materials (Wiley) identifying promising conditions in PEG-based, glycosaminoglycan (GAG)-containing hydrogels: https://lnkd.in/eEaVRHxP 1. 💡 We found that the overall concentration as well as sulfation pattern of the GAG heparin are crucial in instructing 3D microvascular network formation and long-term stability.   2. 🔎 Formed dense and stable microvascular networks further supported mesenchymal stromal cell proliferation, ECM deposition and overall matrix stiffening. 3. 📈 Our systematic study gives great insights for future fine-tuning of stable long-term vascularization in other 3D tissue- and disease models. 👏 Congrats to Yanuar Dwi Putra Limasale and the team Marten Samulowitz, Passant Atallah, PhD, Jana Sievers-Liebschner, Nicholas Dennison, Uwe Freudenberg, Jens Friedrichs and Carsten Werner 🤝 Further support by our colleagues at Leibniz-Institut für Polymerforschung Dresden and funding by Deutsche Forschungsgemeinschaft (DFG) - German Research Foundation is highly appreciated #TissueEngineering #RegenerativeMedicine #Hydrogel

Optimising lipid nanoparticle dimensions through manufacturing processes: in their latest work, Caitlin McMillan, Yvonne Perrie MBE at the University of Strathclyde and collaborators aimed to determine if the phase ratio-to-size relationship applied to two clinically used LNP formulations, those incorporating ALC-0315 and SM-102 as ionizable lipids, and to evaluate how phase ratio and LNP size impact in vitro and in vivo expression https://lnkd.in/ewB6yTrp They found that adjusting the phase ratio influenced LNP size, producing particles ranging from 60 to 140 nm in diameter for further analysis. LNPs larger than 120 nm displayed a distinct morphology, commonly showing a double-lamellar structure in CryoTEM imaging, contrasting with the structure of smaller LNPs. An article co-authored by Amy Druschitz, Steve Rumbelow, Ankita Borah, Burcu Binici and Zahra Rattray #LNPs #characterisation #DLS #NTA #encapsulation #Vesiculab

New Paper on Modulating Paracrine Cell Signals in a Biomimetic Wound Healing Model! 📣 In cooperation with colleagues from the Leibniz-Institut für Polymerforschung Dresden and Max Bergman Center of Biomaterials Dresden, we published a paper in Gels on a biomimetic wound healing model using cocultures of primary human fibroblasts and macrophages in 3D collagen networks functionalized with sulfated glycosaminoglycans. We show that the network functionalization and the degree of sulfation of GAGs influences the paracrine cell-cell signaling in the in vitro model. Have a look in our paper: https://lnkd.in/edYKnAHj #woundhealing #fibroblasts #macrophages #3Dcollage #cellsignaling

A comprehensive kit driving transformation of biomedical research.

Conclusions Since autophagy has been proven to be involved in chemotherapy and radiotherapy, researchers could target the modulation of autophagy by NPs as an attractive new therapy. Researchers investigated Fe3O4 NPs and SiO2 NPs to determine if they might improve kidney cell proliferation and repair kidney injury. The current result illustrated that Fe3O4 and SiO2 NPs could promote cell growth and modulate autophagy. Especially, the mixture of Fe3O4 and SiO2 NPs induces the autophagy process for stimulation of and enhancement of cell growth. We will further investigate the molecular links/pathways between Fe3O4 and SiO2 for their synergistic activity to understand their mechanisms. Indeed, modulating autophagy could help to enhance its therapeutic effects.

🧬 Ready to take organoid research to the next level? Dive into the intricate 3D vascular structures captured through advanced microscopy! 🌱✨This video highlights how our #OrganoidReagentKit enables precise analysis and development of complex tissue models. 🚀 With our innovative solutions, explore how 3D cell culture and organoid technology are transforming biomedical research! 🌐🔬 #OrganoidResearch #3DCellCulture #OrganoidReagentKit #VascularOrganoids #DrugDiscovery #BiotechInnovation #NestBiotech #RegenerativeMedicine #PharmaceuticalResearch

🌟 Thrilled to showcase our newest publication: “Photocrosslinkable Microgels Derived from Human Platelet Lysates: Injectable Biomaterials for Cardiac Cell Culture”! Taking advantage of the exceptional properties of hPLMA (human platelet lysate methacrylate), this research focused on developing microgels to create injectable systems for in situ cell delivery. 𝗵𝗣𝗟𝗠𝗔 𝗺𝗶𝗰𝗿𝗼𝗴𝗲𝗹𝘀, produced using a microfluidics system, 𝗰𝗮𝗻 𝘀𝗲𝗿𝘃𝗲 𝗮𝘀 𝗮𝗻𝗰𝗵𝗼𝗿𝘀 𝗳𝗼𝗿 𝗰𝗮𝗿𝗱𝗶𝗮𝗰 𝗮𝗻𝗱 𝗲𝗻𝗱𝗼𝘁𝗵𝗲𝗹𝗶𝗮𝗹 𝗰𝗲𝗹𝗹𝘀 thus enabling the creation of short-term injectable systems in xeno-free environments. Are you curious about how this could impact cardiac therapy? 🫀 Read the full article here: https://lnkd.in/dRKfuid4 #Biomaterials #CardiacResearch #TissueEngineering #RegenerativeMedicine

Their abstract: Red-shifted bioluminescence is highly desirable for diagnostic and imaging applications. Herein, we report a semisynthetic NanoLuc (sNLuc) based on complementation of a split NLuc (LgBiT) with a synthetic peptide (SmBiT) functionalized with a fluorophore for BRET emission. We observed exceptional BRET ratios with diverse fluorophores, notably in the red (I674/I450 > 14), with a brightness that is sufficient for naked eye detection in blood or through tissues. To exemplify its utility, LgBiT was fused to a miniprotein that binds HER2 (affibody, ZHER2), and the selective detection of HER2+ SK-BR-3 cells over HER2– HeLa cells was demonstrated.

Characterization by SPR of the kinetics of binders to Galectin 1: More great results from the NovAlIX biophysics team. Check out the findings here: https://lnkd.in/d_VQsqCv.

Understanding nanoparticle-liver interactions in nanomedicine https://lnkd.in/dcM4p4DH