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Cover slips biofilm technique This technique focuses on bacterial attachment and accumulation on abiotic surfaces the method is superior for confocal microscopy biofilm visualization. The assays have proven effective at identifying mechanisms involved in cell attachment and biofilm accumulation. Studies have shown that when medical devices are implanted they are coated with host factors, such as matrix proteins, that facilitate S. aureus attachment and biofilm formation (Walker an Horswill; 2012).

What’s the difference between 2D and #3dcellculture? While 2D cell culture is convenient for cell analysis, 3D cell culture has the ability to mimic the exact environment of tissues, decreasing the need for animal testing and providing results that will improve the translation from in-vitro to in-vivo studies. Humabiologics offers native human-derived biomaterials to mimic the native tissue-specific environment for regenerative medicine and tissue engineering applications such as 2D and 3D cell culture that will help develop lifesaving therapies. Learn more about our product offerings here: https://lnkd.in/e-pi48RE #collagen #ISO13485 #gelatin #tissueengineering 

Developing and characterising nanofibrous scaffolds with extracellular vesicles loaded with curcumin: in their latest article, Adrienn Nochta-Kazsoki PharmD, PhD, Dr. Zelkó Romána at Semmelweis University incorporated large curcumin-loaded EVs into fast-dissolving poly(vinyl alcohol) nanofibers through electrospinning, using aqueous PVA solutions. Confocal laser scanning microscopy confirmed the presence of curcumin-loaded lEVs within the nanofibers. https://lnkd.in/gCqubadN Release studies showed high drug concentrations in lEV-containing nanofibers, underscoring the potential of EV-loaded nanofibrous systems for enhanced therapeutic applications and improved patient outcomes. An article co-authored by Krisztina Németh, TAMÁS VISNOVITZ, Dorina Lenzinger and Edit Buzás #extracellularvesicles #exosomes #drugloading #bioengineering #Vesiculab

One step closer to long-term vascularization of 3D in vitro models? 📢 🎉 Our new paper is out in Advanced Functional Materials (Wiley) identifying promising conditions in PEG-based, glycosaminoglycan (GAG)-containing hydrogels: https://lnkd.in/eEaVRHxP 1. 💡 We found that the overall concentration as well as sulfation pattern of the GAG heparin are crucial in instructing 3D microvascular network formation and long-term stability.   2. 🔎 Formed dense and stable microvascular networks further supported mesenchymal stromal cell proliferation, ECM deposition and overall matrix stiffening. 3. 📈 Our systematic study gives great insights for future fine-tuning of stable long-term vascularization in other 3D tissue- and disease models. 👏 Congrats to Yanuar Dwi Putra Limasale and the team Marten Samulowitz, Passant Atallah, PhD, Jana Sievers-Liebschner, Nicholas Dennison, Uwe Freudenberg, Jens Friedrichs and Carsten Werner 🤝 Further support by our colleagues at Leibniz-Institut für Polymerforschung Dresden and funding by Deutsche Forschungsgemeinschaft (DFG) - German Research Foundation is highly appreciated #TissueEngineering #RegenerativeMedicine #Hydrogel

📢 A new Special Issue "Materials and Methods for Retinal Injury and Repair" is now open for submissions! 🥼 This Special Issue is guest edited by Dr. Jesse Sengillo, from Bascom Palmer Eye Institute, University of Miami, USA. 💡 Topics to be covered in this Special Issue include the use of biomaterials for retinal repair, innovative surgical techniques for treating retinal injuries, advancements in drug delivery systems targeting specific retinal layers, and the development of novel imaging modalities for assessing retinal damage and repair. 🔗 Click the link to access more details about the Special Issue: https://lnkd.in/e6yu-qGg. 🕑 Deadline for manuscript submissions is 30 September 2024. 🎉 Welcome to join us as authors and reviewers! 👏 And welcome to follow our LinkedIn account: https://lnkd.in/grPNkBrg. #retinal #injury #repair #biomaterials #surgical #drug_delivery #imaging #stemcell #gene_therapy #regenerative_medicine

The transfer of stem cell colonies is often a stressful procedure resulting in a great number of dead cells. CellCelector can provide a gentle-mechanical transfer of stem cells and stem cell colonies with high specificity, while maintaining their viability, clonality and pluripotent characteristics. #stemcells #stemcellcolonies #lifescience #cellcelector

From 3D organoids to air-liquid interface cultures and “lung-on-chip” devices, each in vitro lung model offers unique insights into different aspects of lung function. Read our blog post to discover the intricacies and possibilities of the various types of in vitro lung models and how to select the right one for your research: https://hubs.li/Q02SWjTP0 #respiratoryresearch #biomedicalresearch

Respiratory diseases are on the rise- explore how in vitro lung models can bring your research to the next Level:

Their abstract: Red-shifted bioluminescence is highly desirable for diagnostic and imaging applications. Herein, we report a semisynthetic NanoLuc (sNLuc) based on complementation of a split NLuc (LgBiT) with a synthetic peptide (SmBiT) functionalized with a fluorophore for BRET emission. We observed exceptional BRET ratios with diverse fluorophores, notably in the red (I674/I450 > 14), with a brightness that is sufficient for naked eye detection in blood or through tissues. To exemplify its utility, LgBiT was fused to a miniprotein that binds HER2 (affibody, ZHER2), and the selective detection of HER2+ SK-BR-3 cells over HER2– HeLa cells was demonstrated.