Joel Walker’s Post

Cover slips biofilm technique This technique focuses on bacterial attachment and accumulation on abiotic surfaces the method is superior for confocal microscopy biofilm visualization. The assays have proven effective at identifying mechanisms involved in cell attachment and biofilm accumulation. Studies have shown that when medical devices are implanted they are coated with host factors, such as matrix proteins, that facilitate S. aureus attachment and biofilm formation (Walker an Horswill; 2012).

Developing and characterising nanofibrous scaffolds with extracellular vesicles loaded with curcumin: in their latest article, Adrienn Nochta-Kazsoki PharmD, PhD, Dr. Zelkó Romána at Semmelweis University incorporated large curcumin-loaded EVs into fast-dissolving poly(vinyl alcohol) nanofibers through electrospinning, using aqueous PVA solutions. Confocal laser scanning microscopy confirmed the presence of curcumin-loaded lEVs within the nanofibers. https://lnkd.in/gCqubadN Release studies showed high drug concentrations in lEV-containing nanofibers, underscoring the potential of EV-loaded nanofibrous systems for enhanced therapeutic applications and improved patient outcomes. An article co-authored by Krisztina Németh, TAMÁS VISNOVITZ, Dorina Lenzinger and Edit Buzás #extracellularvesicles #exosomes #drugloading #bioengineering #Vesiculab

A comprehensive kit driving transformation of biomedical research.

https://lnkd.in/e5wxYs6d #Bioprobes#Lanthanide#Cytosolic Peptide Penetration#Luminescent#Live cells#Two-Photon Microscopy# Abstract : Lanthanide(III) (Ln3+) complexes have desirable photophysical properties for optical bioimaging. However, despite their advantage over organic dyes, their use for microscopy imaging is limited by the high-energy UV excitation they require and their poor ability to cross the cell membrane and reach the cytosol. Here we describe a novel family of lanthanide-based luminescent probes, termed dTAT[Ln·L], based on (i) a DOTA-like chelator with a picolinate moiety, (ii) a two-photon absorbing antenna to shift the excitation to the near infrared and (ii) a dimeric TAT cell-penetrating peptide for cytosolic delivery. Several Tb3+ and Eu3+ probes were prepared and characterized. Two-photon microscopy of live cells was attempted on a commercial microscope with the three probes showing the highest quantum yields (>0.15). A diffuse Ln3+ emission was detected in most cells, which is characteristic of cytosolic delivery of the Ln3+ complex. The cytotoxicity of these three probes was evaluated and the IC50 ranged from 7µM to >50µM. The addition of a single positive or negative charge to the antenna of the most cytotoxic compound was sufficient to lower significantly or suppress its toxicity under the conditions used for two-photon microscopy. Therefore, the design reported here provides excellent lanthanide-based probes for two-photon microscopy of living cells.

🧬 Ready to take organoid research to the next level? Dive into the intricate 3D vascular structures captured through advanced microscopy! 🌱✨This video highlights how our #OrganoidReagentKit enables precise analysis and development of complex tissue models. 🚀 With our innovative solutions, explore how 3D cell culture and organoid technology are transforming biomedical research! 🌐🔬 #OrganoidResearch #3DCellCulture #OrganoidReagentKit #VascularOrganoids #DrugDiscovery #BiotechInnovation #NestBiotech #RegenerativeMedicine #PharmaceuticalResearch

What’s the difference between 2D and #3dcellculture? While 2D cell culture is convenient for cell analysis, 3D cell culture has the ability to mimic the exact environment of tissues, decreasing the need for animal testing and providing results that will improve the translation from in-vitro to in-vivo studies. Humabiologics offers native human-derived biomaterials to mimic the native tissue-specific environment for regenerative medicine and tissue engineering applications such as 2D and 3D cell culture that will help develop lifesaving therapies. Learn more about our product offerings here: https://lnkd.in/e-pi48RE #collagen #ISO13485 #gelatin #tissueengineering 

Spherical nucleic acids (SNAs) are a 3D spherical nanostructure composed of highly oriented, dense layers of oligonucleotides conjugated to a hollow or solid core. This structure allows SNAs to show resistance to nuclease degradation, enter into nearly all cells without transfection agents and enable precise interactions with target molecules. Based on superior biological properties, SNAs can be tailored for diverse biological applications, rendering them a flexible and biosafe tool for biological applications as well as an enabling platform for therapy. In this review, we mainly discuss the structure and conjugation mode of SNAs and focus on recent advances in their applications, such as biomedical detection, imaging, and drug delivery. Finally, the remaining challenges and future directions of SNAs are also discussed and proposed.

One step closer to long-term vascularization of 3D in vitro models? 📢 🎉 Our new paper is out in Advanced Functional Materials (Wiley) identifying promising conditions in PEG-based, glycosaminoglycan (GAG)-containing hydrogels: https://lnkd.in/eEaVRHxP 1. 💡 We found that the overall concentration as well as sulfation pattern of the GAG heparin are crucial in instructing 3D microvascular network formation and long-term stability.   2. 🔎 Formed dense and stable microvascular networks further supported mesenchymal stromal cell proliferation, ECM deposition and overall matrix stiffening. 3. 📈 Our systematic study gives great insights for future fine-tuning of stable long-term vascularization in other 3D tissue- and disease models. 👏 Congrats to Yanuar Dwi Putra Limasale and the team Marten Samulowitz, Passant Atallah, PhD, Jana Sievers-Liebschner, Nicholas Dennison, Uwe Freudenberg, Jens Friedrichs and Carsten Werner 🤝 Further support by our colleagues at Leibniz-Institut für Polymerforschung Dresden and funding by Deutsche Forschungsgemeinschaft (DFG) - German Research Foundation is highly appreciated #TissueEngineering #RegenerativeMedicine #Hydrogel

Check out this recent article from Anna Jezierski from University of Ottawa on single step 3D #bioprinting of alginate–collagen hydrogel fiber rings to promote angiogenic network formation! A new single-step 3D bioprinting method is revolutionizing biofabrication with the Aspect Biosystems RX1 technology. This technique creates intricate vascular structures by layering hydrogel rings embedded with brain endothelial cells, promoting natural angiogenesis and tissue-like network development. This innovative approach can be customized for various endothelial cell types, enhancing our understanding of blood vessel formation and paving the way for advanced tissue models in drug discovery and regenerative medicine. Read more here: https://lnkd.in/eUVsnXcU

👉 New Method for LNP Characterization —— Cryo-Electron Microscopy 👈 LNP stands for Lipid Nano Particle, which refers to lipid nanoparticles composed of biocompatible and biodegradable lipid molecules, similar to cell membranes. Due to their stable physicochemical properties and biological safety, LNPs are widely employed in the field of mRNA drug and vaccine research for drug delivery. Electron microscopy has become a crucial tool for characterizing structures at the atomic and molecular scale in scientific research. Cryo-electron microscopy, as a derivative technique of electron microscopy, involves encapsulating biological samples in a thin layer of vitrified ice. Under electron beam irradiation, the samples remain in their native structure and intrinsic state throughout the imaging process. The quality of LNP (such as encapsulation efficiency, drug loading, and stability) largely depends on the uniformity and stability of its vesicle structure. Cryo-electron microscopy is the only technique that can observe the internal density of LNPs, enabling the detection of encapsulation efficiency or particle filling/void ratio. Parameters such as particle size distribution, membrane thickness, lamellar distribution, overall morphology, etc., are all important indicators for quality control of LNPs. Cryo-electron microscopy allows for rapid and accurate characterization and analysis of various indicators through a single snapshot. Compared to other characterization techniques, cryo-electron microscopy provides a direct and visual observation of samples, making characterization data more convincing. At the same time, the ability to characterize multiple indicators in one go reduces the consumption of LNP samples, thus lowering the cost of characterization and detection. #cryoEM #molecule #drugdiscovery #lipidnanoparticle #LNP #mRNA