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Skip the preliminary experiments and dive straight into the spatial biology of your sample. With 20 markers, the predefined REAplex IO Core panel allows you to visualize and plunge into the tumor microenvironment and see key invading immune cells – no need to do panel establishment and protocol optimization. With the basics already covered you can focus on your research and the specific markers you want! The kit even includes a guideline and template for data analysis to turn the stunning images of your favorite cells into meaningful plots. Take the plunge 👉 https://ow.ly/JkqB50UcsO3

Learn firsthand how to perform whole slide imaging of 40+ protein markers at unprecedented speed. Join us for a webinar with the AFC Association Française de Cytométrie on April 23 that will showcase a new imaging workflow to identify key cell populations and the spatial context of their interactions in complex tissues. Also hear about recent publications using this approach. Sign in here: https://lnkd.in/gPFja9pE #tissueimaging #ImagingMassCytometry #themoreyouknow

Implementing confocal spectroscopy to assess payload heterogeneity in lipid nanoparticles: Yizong Hu, Tine Curk, Hai-Quan Mao, Jeff Tza-Huei Wang at The Johns Hopkins University and collaborators introduced cylindrical illumination confocal spectroscopy combined with in-line single-nanoparticle free solution hydrodynamic separation to simultaneously characterise the size of RNA-LNPs and the loading levels of RNA, helper lipids, and PEGylated lipids at the single-nanoparticle level https://lnkd.in/eXendpEt They observed that correlating LNP size with payload provided insights into LNP structure and RNA distribution, revealing significant heterogeneity in payload distribution even among LNPs of the same size. An article also authored by Sixuan Li, Jinghan Lin, Zachary Schneiderman, Fangchi Shao, Lai Wei, Andrew Li, Kuangwen Hsieh and Efrosini Kokkoli #LNP #nanoparticles #spectroscopy #drugdelivery #payload #Vesiculab

New webinar upcoming! Join us on May 15th to hear Dr. Sonja Lorenz talk about her work on the HACE1 ubiquitin ligase, and the role #MassPhotometry played in it!  Ubiquitin ligases (E3) are central players in the regulation of a myriad cellular pathways. In this study, Dr. Lorenz’s group used cryo-EM and biophysical analyses to study the mechanisms of regulation and specificity of the HACE1 ubiquitin ligase.  Mass photometry was helpful by providing key information on the oligomerization behavior of different mutational variants at low concentrations.   Register now for the webinar and hear the full story 👇 👇  https://lnkd.in/dgQGqEnT

New mass spectrometry imaging (MSI) method of brain organoids allows mapping metabolite distributions – offering insights into brain metabolic pathways and metabolite signatures during early development and in disease. Read more now 👉 https://ow.ly/8yY350TPl6I

📆Thursday, August 22nd ⏰7AM & 10AM PDT / 4PM & 7PM CEST BACKGROUND 👀 Image-enhanced flow cytometry is a rapidly growing technology which allows biologists to capture both traditional flow cytometry data and morphological parameters in a single sample acquisition. In this webinar, you will learn: 🔴How image-enhanced flow cytometry works 🟡How we can use the automated image analysis and high-speed data acquisition of the Invitrogen™ Attune™ CytPix™ Flow Cytometer to take a deep dive into several unique populations 🟢A variety of applications that have been improved or made possible through image-enhanced flow cytometry 🔵How image analysis provided important insights on cell health and morphology post-sorting 👉Don’t miss out and register today! #thermofisheremp

𝐀𝐩𝐩𝐥𝐲𝐢𝐧𝐠 𝟑𝐃 𝐜𝐨𝐫𝐫𝐞𝐥𝐚𝐭𝐢𝐯𝐞 𝐬𝐭𝐫𝐮𝐜𝐭𝐮𝐫𝐞𝐝 𝐢𝐥𝐥𝐮𝐦𝐢𝐧𝐚𝐭𝐢𝐨𝐧 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 𝐚𝐧𝐝 𝐗-𝐫𝐚𝐲 𝐭𝐨𝐦𝐨𝐠𝐫𝐚𝐩𝐡𝐲 𝐭𝐨 𝐜𝐡𝐚𝐫𝐚𝐜𝐭𝐞𝐫𝐢𝐬𝐞 𝐡𝐞𝐫𝐩𝐞𝐬 𝐬𝐢𝐦𝐩𝐥𝐞𝐱 𝐯𝐢𝐫𝐮𝐬-𝟏 𝐦𝐨𝐫𝐩𝐡𝐨𝐠𝐞𝐧𝐞𝐬𝐢𝐬 This recently published preprint highlights the benefits of employing multimodal correlative imaging in characterising how viral genes are involved in the assembly of herpes simplex virus-1 (HSV-1). Dr Kamal Nahas and colleagues from the Diamond Light Source in the UK, along with co-authors from the University of Cambridge, Oxford and Colombo have generated some insightful images of cell structure that show the HSV-1 virus particles at different stages of assembly. By performing a comparative analysis of nine HSV-1 mutants lacking specific structural proteins, the research study reveals the relative roles of each viral protein in virus assembly. These findings highlight the utility of 𝐜𝐨𝐫𝐫𝐞𝐥𝐚𝐭𝐢𝐯𝐞 𝐜𝐫𝐲𝐨-𝐟𝐥𝐮𝐨𝐫𝐞𝐬𝐜𝐞𝐧𝐜𝐞 𝐩𝐥𝐮𝐬 𝐜𝐫𝐲𝐨-𝐬𝐨𝐟𝐭-𝐗-𝐫𝐚𝐲 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 (𝐂𝐋𝐗𝐌) for probing trajectories of intracellular pathogen assembly. Read the full paper here: https://lnkd.in/g9iXZCUP #HSV1 #ViralAssembly #ResearchBreakthrough #CLXM #SXT #FM #Virus

[NEW] Is aging constant or do we age in stages?! Researchers at Leibniz-Institut für Verbundwerkstoffe GmbH (IVW) recently epigenetically analyzed mouse colon tissues to reveal non-linear DNA methylation patterns during #aging that highlight critical stages in this all-important process! ➡ https://bit.ly/3QuwiTS

𝐀𝐩𝐩𝐥𝐲𝐢𝐧𝐠 𝟑𝐃 𝐜𝐨𝐫𝐫𝐞𝐥𝐚𝐭𝐢𝐯𝐞 𝐬𝐭𝐫𝐮𝐜𝐭𝐮𝐫𝐞𝐝 𝐢𝐥𝐥𝐮𝐦𝐢𝐧𝐚𝐭𝐢𝐨𝐧 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 𝐚𝐧𝐝 𝐗-𝐫𝐚𝐲 𝐭𝐨𝐦𝐨𝐠𝐫𝐚𝐩𝐡𝐲 𝐭𝐨 𝐜𝐡𝐚𝐫𝐚𝐜𝐭𝐞𝐫𝐢𝐬𝐞 𝐡𝐞𝐫𝐩𝐞𝐬 𝐬𝐢𝐦𝐩𝐥𝐞𝐱 𝐯𝐢𝐫𝐮𝐬-𝟏 𝐦𝐨𝐫𝐩𝐡𝐨𝐠𝐞𝐧𝐞𝐬𝐢𝐬 This recently published preprint highlights the benefits of employing multimodal correlative imaging in characterising how viral genes are involved in the assembly of herpes simplex virus-1 (HSV-1). Dr Kamal Nahas and colleagues from the Diamond Light Source in the UK, along with co-authors from the University of Cambridge, Oxford and Colombo have generated some insightful images of cell structure that show the HSV-1 virus particles at different stages of assembly. By performing a comparative analysis of nine HSV-1 mutants lacking specific structural proteins, the research study reveals the relative roles of each viral protein in virus assembly. These findings highlight the utility of 𝐜𝐨𝐫𝐫𝐞𝐥𝐚𝐭𝐢𝐯𝐞 𝐜𝐫𝐲𝐨-𝐟𝐥𝐮𝐨𝐫𝐞𝐬𝐜𝐞𝐧𝐜𝐞 𝐩𝐥𝐮𝐬 𝐜𝐫𝐲𝐨-𝐬𝐨𝐟𝐭-𝐗-𝐫𝐚𝐲 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲 (𝐂𝐋𝐗𝐌) for probing trajectories of intracellular pathogen assembly. Read the full paper here: https://lnkd.in/g9iXZCUP hashtag #HSV1 #ViralAssembly #ResearchBreakthrough #CLXM #SXT #FM #Virus