Danaher Life Sciences’ Post

Developing and screening plasmid libraries is critical to biotherapeutic development. Maintaining plasmid libraries and preventing evolution is essential for consistency. Learn how we can help: https://bit.ly/4ddFg15 #DanaherLifeSciences #pDNA #plasmid #drugdiscovery #drugdevelopment

Our LNP Encapsulation Kits simplify the preparation of lipid nanoparticles for different biomedical uses. Key Features: - Complete Kit: Contains ionizable lipids, base lipids, long-circulating lipids, and cholesterol. - Multifunctional: Suitable for encapsulating RNAs, proteins, and drugs. - Performance: Ensures biocompatibility and efficient encapsulation. - Wide Applicability: Ideal for cell transfection and animal studies. https://lnkd.in/gZ8ERAt5

𝗧𝗮𝗰𝗸𝗹𝗶𝗻𝗴 𝗚𝗹𝘆𝗰𝗮𝗻 𝗔𝗻𝗮𝗹𝘆𝘀𝗶𝘀 𝗳𝗼𝗿 𝗠𝘂𝗹𝘁𝗶𝘀𝗽𝗲𝗰𝗶𝗳𝗶𝗰 𝗔𝗻𝘁𝗶𝗯𝗼𝗱𝗶𝗲𝘀! A new article by Cynthia Challener in BioPharm International in discusses the increasing complexity of glycan analysis for multispecific antibodies and why this is essential for drug safety and efficacy. Featuring insights from #TeamSCIEX member Paula Orens, the article explores the role of technologies like liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry (MS) in overcoming these challenges. 𝗥𝗲𝗮𝗱 𝗠𝗼𝗿𝗲 👉 https://bit.ly/3ZVDqy2 #GlycanAnalysis #MultispecificAntibodies #SCIEX #MassSpec #BioPharmInternational #DanaherNews

Canton Biologics' DTEasy (Difficult to Express) platform is distinguished by its efficacy and versatility. We provide a comprehensive range of vectors meticulously engineered to address varying levels of expression difficulty. 📌 In one instance, regarding tissue plasminogen activator (t-PA): Challenges we faced: 1) the non-covalent complex formed between t-PA and its inhibitor exhibited a loosely structured nature, facilitating separation; 2) the traditional purification process for t-PA generally requires three to four steps to achieve purity exceeding 90%. Solutions: We developed a dual expression vector that co-expresses t-PA in conjunction with its inhibitor, complemented by an efficient one-step chromatographic purification process. Results: The final purified yield of t-PA reached an impressive 361.5 mg/L, surpassing current technological benchmarks by more than threefold. 📈 📩 Contact us: BD@cantonbio.com #BiotechInnovation #DTEasyPlatform #ProteinExpression 

Exciting advancements in bioprocessing! Discover how Repligen's #ProStream and #HyStream tangential flow filtration cassettes are enhancing efficiency and reliability in antibody-drug conjugate (ADC) production. These cutting-edge technologies offer superior performance, ensuring high-quality outcomes in complex ADC processes. Let's embrace innovation in biomanufacturing! #Bioprocessing #Innovation #ADC #Repligen

NEXT WEEK! DDL 2024 CONFERENCE POSTER: The Evaluation of Equivalence of Mometasone Furoate Nasal Sprays via an In-Vitro Pathway using Dissolution, MDRS and Laser Diffraction techniques. Not attending #DDL2024? Request a copy now: https://lnkd.in/dpnKm3g Authors: Lily Richards, Darren Long, Mervin Ramjeeawon, Hannah Stafford & Yuen Ting Wong In May 2023, the FDA updated the Fluticasone Propionate, Mometasone Furoate and Fluticasone Furoate nasal suspensions FDA bioequivalence guidelines to include dissolution testing as an in-vitro Bioequivalence (IVBE) requirement. This update has given generic companies the opportunity to understand better the product being formulated by detecting meaningful differences between the candidate Test and Reference product formulations. The differences between the products may be minor however the knowledge gained from the dissolution testing can be further expanded on by using other orthogonal techniques, such as Morphology Directed Raman Spectroscopy (MDRS) and Particle Size Distribution (PSD) by Laser Diffraction. These additional tests can give insights into the reasons for differences seen in the dissolution data by assessing the particle size within the bulk product formulation, whilst also giving API specific size and shape information, which are critical variables that will affect dissolution (and as a result for these products, the pharmacokinetic profile observed). This design of the experimental approach is not limited to nasal sprays and can also be used to aid in de-formulation, early screening and assessment of other OINDP delivery platforms. In this study, the dissolution profiles of two commercially available Mometasone Furoate nasal sprays, which have already been proven to be bioequivalent, were appraised, and differences between these two products were further assessed using MDRS and Laser diffraction to investigate and determine the reasons behind any differences seen. #Intertek #Nasal #DrugDelivery #DrugDevelopment #Pharmaceutical

Utilizing hot-stage polarized microscopy and ATR-FTIR for ramipril co-crystal screening, supported by principal component analysis and cluster analysis. https://ow.ly/m8xz50Qx2zK #PharmaceuticalTechnology #Pharmacy #DrugQuality

Learn about measuring thermal stability, particle size, and polydispersity using the Zetasizer and NanoSight in this Lentivirus case study! Read it here 👇 We used the Zetasizer Advance Ultra Red and NanoSight Pro to investigate colloidal and morphological changes to the lentivirus structure after different storage conditions. Learn about: How the NanoSight Pro uses nanoparticle tracking analysis to measure nanoparticle size and concentration 🧪 How the Zetasizer Advance uses dynamic light scattering (DLS) to measure particle concentration and colloidal stability 💪 The role thermal stability can play in improving long-term stability of pharmaceuticals 💊 #Pharma #DLS #NTA

We’re thrilled to share our latest white paper, which explores how SK pharmteco has evolved the HEK293 cell line to become a game-changer in viral vector manufacturing. 🔬 This comprehensive resource examines how we've enhanced these cells to boost titers and significantly reduce manufacturing costs. Key highlights include: • 𝗢𝗽𝘁𝗶𝗺𝗮𝗹 𝗖𝗲𝗹𝗹𝘀: Discover the importance of selecting the right cells lines for generating CGMP Master Cell Banks. • 𝗧𝗲𝗰𝗵𝗻𝗶𝗰𝗮𝗹 𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝗺𝗲𝗻𝘁𝘀: Learn about the technical challenges and solutions in developing a high-performing, monoclonal suspension HEK293 cell line for CGMP cell banking. • 𝗜𝗺𝗽𝗮𝗰𝘁 𝗼𝗻 𝗣𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝗼𝗻: Our CGMP Serum-free Suspension Cell Line for Universal AAV, LVV, and Ad vectors Production is simplifying the delivery of Advanced Therapies. Whether you're involved in bioprocessing, or viral vector production, this white paper is an invaluable resource for enhancing quality of vector preps, overall scalability, and lowering COGS of your CGMP vector. 🔗 Click the link in the comments to download the white paper and stay ahead in the rapidly evolving world of viral vector manufacturing. #ViralVector #GeneTherapy #Bioprocessing #AAV #LVV #Adeno #Vector #CGMP #Biotech

Preprint alert! 🚨 "To be protonated or not to be protonated? That is the question! 🤔 In our latest study, we used constant-pH simulations to uncover the dynamic (de)protonation of ionizable aminolipids in Lipid Nanoparticles (LNPs). 🧪 With hundreds of titratable sites, we explored how these key components behave in the BioNTech & Pfizer LNP formulation. Our findings reveal a fascinating pKa shift by more than 3 units and resolve a position-dependent protonation of aminolipids. #BioMemPhys #membranes #LNP #FAU https://lnkd.in/eQHEE99Z