ISS inc.’s Post

Interesting LNP data coming out of the Thomas L. Andresen lab at the Technical University of Denmark. 💥 Roughly only 12% of mRNA-LNPs are "empty" ⭕ This value is even lower for siRNA (5.8% empty) and much higher for plasmid DNA (26.4% empty), indicating the cargo size plays a major role in the amount of cargo encapsulation. They tested this across four well known LNP standards and obtained similar results. 𝐂𝐨𝐧𝐬𝐞𝐪𝐮𝐞𝐧𝐜𝐞𝐬? A vast majority of LNPs in clinically relevant formulations are encapsulating nanomedicines and are thus able to exert their therapeutic activities. Would be interested to verify these results, obtained using single particle fluorescence confocal microscopy, with other orthogonal methods. 👇 Link to the research article below: https://lnkd.in/gymebH-E #LNP #LNPs #lipidnanoparticles #mRNA #HelixBiotech

Simplifying single extracellular vesicle imaging: in their latest article, Andras Saftics, Ph.D., Tijana Jovanovic-Talisman at City of Hope and collaborators developed a novel method, called the SEVEN-Universal Protocol (SEVEN-UP) to democratise single EV studies by combining super-resolution radial fluctuations (SRRF) microscopy with advanced data analysis. SRRF is compatible with various microscopes and fluorophores, making it widely accessible. By integrating affinity isolation with SRRF microscopy and machine learning-supported analysis algorithms, they achieved precise EV quantification. They used this method to assess RNA content in EVs from conditioned cell culture media https://lnkd.in/gy_hbQsc Additionally, using SYTO RNASelect dye, they found that 18% of EVs from HEK 293T cells contained RNA, and these RNA-containing EVs were significantly larger than the general EV population. Overall, their approach provides an economical, multiparametric method for single EV characterisation, offering valuable tools to the research community. An article co-authored by Benjamin Purnell, Bálint Béres, S. Thompson, Nan Jiang, Ima Ghaeli, Carinna Lima, Brian D Armstrong PhD and Kendall Van Keuren-Jensen #extracellularvesicles #exosomes #fluorescence #microscopy #Vesiculab

This has been one of my most favorite project to have worked on during my PhD & it is now finally out!!!(well, was out 3 months ago already) It is also my first, first-author work and has a pun in the title(how cool is that!!!) which makes it extra special! Check it out!!!! https://lnkd.in/dr_2C4Eh TLDR; This study should caution researchers who perform live-cell time-lapse experiments to be aware about sample health, phototoxicity and image acquisition routines. These data also strongly emphasize the crucial importance of exact knowledge on the molecular composition of reagents, buffers and media. The practice that recipes are kept secret and kits are offered as black-boxes solely for commercial benefit is simply not in accordance with good scientific practice which aims at a full, exact and reproducible description of experiments. And last but not the least, this study shows that in this case, confocal TCSPC provides the more reliable data needed to study response kinetics. #microscopy #livecellimaging #FLIM #FRET #Radical #cellsignaling #dynamics #cellbiology #confocal #widefield

📚 Seeing is believing: Download this complimentary Article Collection today! 🔬 Imaging is one of the foundational techniques used in the life sciences. From imaging the expression of individual proteins or genes in single cells to the screening of drugs in multi-well plates, microscopy platforms for cellular imaging are integral tools for unravelling the complexities of biological systems. 🙋♀️ This free article collection from Wiley showcases a diverse range of studies that employ our EVOS Cell Imaging Systems (Thermo Fisher Scientific). What You Will Learn: 👉 Exploring K5 peptide’s role in improving protein delivery into cells 👉 Identifying cell receptors using fluorescent ligands 👉 Assessing crosslinking effects on collagen-elastin scaffolds for wound healing 👉 Investigating miRNAs’ effects on GCTB cell proliferation and movement 👉 Versatility of EVOS Cell Imaging Systems across multiple research fields 🤓 More info: https://lnkd.in/epkBGb5u #EVOS #imaging #thermofisherscientific

Discover daily Biophysics insights on ScienceCast.org, a member of arXiv Labs. In today's digest, explore Shahid et al.'s study on the internal unwinding mechanism of replicative helicases through their own audio pitch. Connect with pioneers and stay ahead in the field. Learn more: https://lnkd.in/evbEVGi6 #Biophysics #ResearchUpdates #ProfessionalNetworking These posts now emphasize the unique feature of arXiv Labs, offering authors a platform to enhance their research visibility through audio pitches, and maintain the tailored approach for each social media platform.

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimised for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging

It was a pleasure to speak at KUL/VIB today, sharing insights on the recent advances of RNAscope technology for whole mount samples 🧠! RNAscope, a next-generation in situ hybridization technology, allows for the detection of single-molecule RNA with unparalleled sensitivity and specificity. While initially optimized for sections, new protocols now enable RNA and protein staining in a variety of 3D/Whole Mount samples like organoids, DRG, or tumors. Researchers are increasingly turning to RNAscope for 3D/Whole mount staining for these key reasons: 🔬 Comprehensive Visualization: Capture the complete architecture of intricate tissues or organoids while preserving spatial relationships crucial for a holistic view in heterogenous samples. 🎯 Enhanced Accuracy: Whole mount staining eliminates the need to reconstruct 2D images into 3D models, providing a more precise representation of cellular interactions and distributions. ⏰ Time Efficiency: Reduce the labor-intensive process of sectioning and speed up assay development. Contrary to antibodies, RNAscope requires minimal optimization for new targets. Thank you to all attendants for the interest and engaging discussions ! If you are curious to learn more or are considering embarking on a project involving 3D/Whole mount samples, feel free to reach out 📢 #RNAscope #Organoid #WholeMount #biotechne

New publication alert in Small Wiley🎊 Our portfolio of nanosensors is expanding! Here, we present dynamic color-switching fluorescent DNA-silver nanoclusters (DNA/AgNCs) to probe the redox state of cells by enabling biosensing and bioimaging of reactive oxygen species (ROS) levels in cells. Thanks to the excellent work by Y Hari Chandana Riddhi Nagda, PhD and coauthors. The outcome is the result of the excellent #interdisciplinary collaboration with Peter Waaben Thulstrup Morten Bjerrum Won Ho Yang and seong wook yang groups from Roskilde University Department of Chemistry, University of Copenhagen, and Yonsei University The redox regulation, maintaining a balance between oxidation and reduction in living cells, is vital for cellular homeostasis, intricate signaling networks, and appropriate responses to physiological cues. Probing the redox state of cells can reveal a lot about the cellular state and can provide new insights into diseases such as cancer and diabetes. Here, we exploited the red-blue emission switching ability of DNA-silver nanoclusters, in response to redox state, to detect and visualize the reactive oxygen species (ROS) levels in cells. The fundamental investigation of the biochemistry of DNA templates and the chemical composition of AgNCs allowed us to develop a more sensitive DNA nanosensor, harboring both AgNCs and organic fluorophores. We hope the ease of design will make our DNA nanosensor more accessible to researchers interested in investigating the dynamics of ROS levels in diseases. https://lnkd.in/dNFcKF2B #RedoxBiology #OxidativeStress #RedoxSensor #DNANanotechnology #Biosensing #SilverNanoclusters #bioimaging #ROSDetection #Nanosensors #Bioimaging

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging #thermofisherscientific

Introducing Invitrogen™ Aluora™ Spatial Amplification Kits 🔬 We are thrilled to announce the availability of our new Invitrogen™ Aluora™ Spatial Amplification Kits! These innovative IHC reagents combine bright Aluora dyes with enzyme-mediated signal amplification to produce covalently attached fluorophores optimized for up to 8-plex spatial imaging. Why choose Aluora™ Spatial Amplification Kits? 🌟 Enhanced Brightness: Bright fluorophores optimized for spatial proteomics applications 🎯 Rare Target Detection: Overcome autofluorescence with enzyme-mediated signal amplification 🌈 Multiplexing Made Easy: Completely covalent labeling allows the use of primary antibodies from the same host species Maximize your IHC experiments today: https://lnkd.in/dMJcNZPU #AluoraSpatialAmplification #Invitrogen #SpatialBiology #MultiplexIHC #FluorescenceImaging