Quantabio’s Post

Tiny tubes, huge impact. Our PCR consumables enable science that changes lives. Learn more: https://shorturl.at/kyDZ8 #QuantitativePcr #RealTimePcr #PcrConsumables #PcrReagents #PcrInstruments #PcrSensitivity #PcrAccuracy #PcrContamination #PcrAutomation #HighThroughputPcr

Tiny tubes, huge impact. Our PCR consumables enable science that changes lives. Learn more: https://shorturl.at/kyDZ8 #QuantitativePcr #RealTimePcr #PcrConsumables #PcrReagents #PcrInstruments #PcrSensitivity #PcrAccuracy #PcrContamination #PcrAutomation #HighThroughputPcr

#Article La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells by Sara Knezevic and Dalibor Stanković https://lnkd.in/gZSTGNMP   #MDPI #carbon #Biochemical #cell #biosensors #sensors #openaccess   #Abstract This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.

No dye? No label? And how do you tag it? Single cell analysis – Label free! Impedance Flow Cytometry (IFC) is a label free technology for single cell analysis. Unlike optical methods, IFC does not need labels, markers or fluorochrome dyes for the identification of viable and dead cells or to study cell metabolic and health status.   Impedance Flow Cytometry goes beyond cell counting and even beyond analysis of dead and viable cells. IFC allows to detect the health status of cells in fermentation processes or to monitor the metabolic status of cell cultures during the production of proteins or other metabolites. And that all without markers or dyes!   Interested in how it works without labels and dyes? More information about single cell analysis based on Impedance Flow Cytometry you can find here: https://lnkd.in/esv6767J   #flowcytometry #cellanalysis #labelfree

Very useful assay developed by Celtarys Research for those working on #GPCR , replace radioactivity with #fluorescence - #medchem #medicinalchemistry #greenchemistry

Explore the benefits and advances in magnetic induction technology in qPCR systems. Our latest editorial discusses the important features like rapid heating, uniform temperature control, and portability advantages over traditional block-based cyclers. https://lnkd.in/gSEG4nQZ

The light in the dark: what you can see from single cells in turbid or opaque media Single-cell analysis is predominantly performed with instruments based on optical methods. Turbidity, opacity, and the occurrence of autofluorescence from the media under investigation can have a detrimental effect on the measurement result or even completely prevent a measurement. Obviously, you can’t see in the dark.   Impedance Flow Cytometry (IFC) proves to be the alternative in this case.  IFC measures electrical cell properties and is not based on optical methods. The presence of turbidity, opacity, or autofluorescence therefore has no impact on the measurement.   Typical applications are: - Turbid solutions: highly concentrated cell suspensions in fermentation - Opaque media: determination of somatic cells in milk or bacterial contaminations in emulsions - Auto-fluorescent media: measurement of microalgae producing metabolites like lipids   Interested in how it works with cell analysis in turbid, opaque, or auto-fluorescent media? More information you can find here: https://lnkd.in/esv6767J      #flowcytometry #cellanalysis #algae #emulsion

Scaling down single-cell reactions to the nanoliter level is critical to minimize the risk of contamination, increase reaction efficiency, and reduce costs.

🧬DBscript Reverse Transcriptase: Superfast protocol with less pipetting steps! 🧬 Looking for reliable and efficient cDNA synthesis? Our Reverse Transcriptase is the ideal choice for both two-step and one-step RT-PCR, designed to enhance your research outcomes. Key Benefits: 🚀 Enhanced Yield: MMLV mutant with reduced RNase H activity ensures more cDNA synthesis and allows for longer cDNAs (> 9 kb). 🌡️ Flexible Temperatures: Perform reverse transcription efficiently at temperatures ranging from 37°C to 60°C, thanks to increased thermostability and our unique buffer formulation. ⏩ Quick Results: Optimized short protocol (1-10 min) for two-step RT-PCR and tailored conditions for one-step RT-PCR. 📦 Complete Kit: Comes with a 5X RT buffer, including dNTPs and a separate DTT solution, to streamline your setup. Elevate your experiments with our top-tier Reverse Transcriptase. Experience improved performance and results in your lab today! Explore more and order now! https://lnkd.in/eBF4hMPj Or contact Markéta Stará and Aleš Pauk for more information! #Biotechnology #LifeSciences #Research #LabLife #cDNASynthesis #RTPCR

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