It's tomorrow. Can't wait. Here is my poster abstract.
Title: Why brain slices should be used as part of the developmental pipeline when screening a drug’s activity in the central nervous system
Due to their relatively inexpensive and high-throughput workflow, in vitro assays are relied upon to predict toxicity and efficacy. However, concerns have been raised over the reliability of the cell-based assays frequently used in drug candidate and toxicity screening. In particular, these assays typically have cells of the same type cultured in a monolayer, which limits cell-cell interaction and cellular differentiation. As well, such models fail to reflect the cellular diversity and microenvironment that exists in vivo, which may significantly alter how cells respond to a drug. Tissue-based models, on the other hand, retain the cellular diversity and architectural complexity of the in vivo microenvironment, which should generate results that more accurately reflect a drug’s behaviour. To illustrate the possibility that a compound may behave differently in cell-based and tissue-based models, I used an example of the latter type (acutely prepared brain slices) to examine an effect that has previously been observed when dissociated neurons are treated with the stress hormone corticosterone. In particular, while an increase in the cell-surface localization of AMPA receptors over time was observed after corticosterone treatment in dissociated neurons, these receptors were downregulated in brain slices. My findings are among several examples that encourage the addition of brain slices in the drug development process, especially for drugs involving the central nervous system. Building on these observations, a method to operationalize the use of brain slices for high-throughput screening using a multi-well format will be discussed.
Master's and PhD students, we want to showcase your research!
CBB, alongside the NSERC CREATE Training in Global Biomedical Technology Research and Innovation, The Office of Research Health Initiatives, and the Biomedical Engineering Graduate Program (BME), is hosting Biomedical Engineering and Technology Research Day on July 24, 2024.
This event is dedicated to showcasing the incredible Biomedical Engineering and Health Technology discoveries of our graduate students. The event will feature a dynamic poster symposium, a thought-provoking keynote address, ample opportunities for networking and collaboration and a chance to win up to $1000!
Visit our website for more information and to register today: https://lnkd.in/gSBwnVsV
#CBB #HealthTechnology #HealthResearch #BiomedicalEngineering #Engineering
![Biomedical Engineering and Technology Research Day | Centre for Bioengineering and Biotechnology]()
Biomedical Engineering and Technology Research Day | Centre for Bioengineering and Biotechnology
uwaterloo.ca
Innovation & Ecosystem | Strategic Partnership Builder | Consultant & Collaborator
3wNICE! Congratulations! Look forward to seeing their efforts to build their (and the state's) research commercialization capacity!